is a prostate-specific transcription factor that is regulated by the androgen receptor in the presence of androgens. It functions as a tumor suppressor against the development of prostatic intraepithelial neoplasia and primary prostate tumors. | Turkish Journal of Biology Turk J Biol (2014) 38: 640-647 © TÜBİTAK doi: Research Article binding to GPX2, QSCN6, SOD1, and SOD2 promoters contributes to antioxidant response regulation via transactivation 1, 2 Bilge DEBELEÇ BÜTÜNER *, Kemal Sami KORKMAZ Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Ege University, Bornova, İzmir, Turkey 2 Department of Bioengineering, Cancer Biology Laboratory, Faculty of Engineering, Ege University, Bornova, İzmir, Turkey 1 Received: Accepted: Published Online: Printed: Abstract: is a prostate-specific transcription factor that is regulated by the androgen receptor in the presence of androgens. It functions as a tumor suppressor against the development of prostatic intraepithelial neoplasia and primary prostate tumors. Here, a recognized approach combining in silico analysis and chromatin immunoprecipitation (ChIP) was used to identify the genes directly regulated by promoter binding in LNCaP cells. Quantitative PCR using ChIP-captured DNAs as templates verified a subset of binding motifs. Thus, in the presence of androgens, significant binding occurs to promoters of GPX2, QSCN6, SOD1, and SOD2 genes that contribute to oxidative stress regulation. Our data demonstrate that is found in a DNA-bound state transiently at a basal level even in the absence of androgens; an increase in androgens promotes binding, perhaps temporally rather than spatially, to the specific sites. The overall changes potentiate the transcriptional regulatory activity of , although they are dependent on the androgen receptor for the target promoters. The results suggest that contributes to an antioxidant response by regulating the transcription of oxidative stress regulators by direct promoter binding. Key words: Chromatin immunoprecipitation, promoter binding, .
