Iberian Buxus spp. are represented by B. sempervirens, restricted to the Cantabrian, eastern Iberian Peninsula, and northeastern mountain systems, emerging scarcely in Portugal, and by B. balearica that occurs in the Betic deep river valleys and in the Balearic islands. | Turkish Journal of Botany Turk J Bot (2016) 40: 1-16 © TÜBİTAK doi: Research Article Biogeographic divergences in the Iberian flora. A morpho-anatomic, ISSR-based, and environmental study of Iberian Buxus sempervirens L. 1 2 1,3 1,3 4 5 Márcia CARVALHO , João ROCHA , Valdemar CARNIDE , Sandra MARTINS , Maurici MUS , Francisco AMICH , 2 6 6 6 6, Rubim ALMEIDA , Cláudia MACHADO , Berta GONÇALVES , Eunice BACELAR , António L. CRESPÍ * 1 Institute for Biotechnology and Bioengineering, Centre of Genomics and Biotechnology (IBB/CGB-UTAD), University of Trás-osMontes e Alto Douro, Vila Real, Portugal 2 Department of Botany, Faculty of Sciences, University of Porto, Porto, Portugal 3 Department of Genetics and Biotechnology, University of Trás-os-Montes e Alto Douro, Vila Real, Portugal 4 Centre d’Estudis de Postgrau, University of Balearic Islands, Palma de Mallorca, Spain 5 Evolution, Taxonomy and Conservation Group (ECOMED), Department of Botany, University of Salamanca, Salamanca, Spain 6 Centre for the Research and Technology of Agro-Environmental and Biological Sciences, CITAB, University of Trás-os-Montes and Alto Douro, UTAD, Quinta de Prados, Vila Real, Portugal Received: Accepted/Published Online: Final Version: Abstract: Iberian Buxus spp. are represented by B. sempervirens, restricted to the Cantabrian, eastern Iberian Peninsula, and northeastern mountain systems, emerging scarcely in Portugal, and by B. balearica that occurs in the Betic deep river valleys and in the Balearic islands. The genetic affinity existent between fifteen populations of B. sempervirens was evaluated by seven ISSRs primers. Moreover, leaf morphoanatomical measurements indicative of leaf performance (such as leaf area, leaf mass per unit area, width, length, shape factor, tissues thickness, stomatal density, and quantification of epi- and intracuticular waxes) and environmental .
