Efficient micropropagation and root culture protocols were developed for the endemic Astragalus chrysochlorus Boiss. & Kotschy. A high frequency of shoot formation (100%) and maximum multiplication (13 shoots per hypocotyl explant) were achieved on Murashige and Skoog (MS) media supplemented with mg/L trans-Zeatin riboside (ZR). | S. HASANÇEBİ, N. TURGUUT KARA, Ö. ÇAKIR, Ş. ARI Research Article Turk J Bot 35 (2011) 203-210 © TÜBİTAK doi: Micropropagation and root culture of Turkish endemic Astragalus chrysochlorus (Leguminosae) Semra HASANÇEBİ1,*, Neslihan TURGUT KARA2 Özgür ÇAKIR2, Şule ARI2,3 1 The Scientific and Technological Research Council of Turkey (TÜBİTAK), Marmara Research Center (MAM), Institute for Genetic Engineering and Biotechnology (GMBE), , Kocaeli - TURKEY 2 İstanbul University, Faculty of Science, Department of Molecular Biology and Genetics, 34118 Vezneciler, İstanbul - TURKEY 3 Research and Application Center for Biotechnology and Genetic Engineering, 34118 Vezneciler, İstanbul - TURKEY Received: Accepted: Abstract: Efficient micropropagation and root culture protocols were developed for the endemic Astragalus chrysochlorus Boiss. & Kotschy. A high frequency of shoot formation (100%) and maximum multiplication (13 shoots per hypocotyl explant) were achieved on Murashige and Skoog (MS) media supplemented with mg/L trans-Zeatin riboside (ZR). By using single regenerated hypocotyl explants, rooting was achieved at a rate of 93% on MS medium containing 2% sucrose without growth regulators. High frequency callus initiation and growth were achieved when single hypocotyl explants were inoculated on MS medium supplemented with mg/L 2,4-Dichlorophenoxyacetic acid. Plant regeneration through indirect organogenesis was achieved on MS medium supplemented with mg/L ZR. Root cultures were successfully established in liquid MS medium containing mg/L α-Naphthaleneacetic acid. The optimised in vitro propagation, callus culture, and root culture protocols offer the possibility to use cell/root culture techniques for vegetative propagation and secondary metabolism studies on Astragalus L. species. Key words: Astragalus, endemic, regeneration, organogenesis, plant conservation Türkiye endemiklerinden Astragalus .
