In this study, ultrastructural changes in root tip cells and leaf cells of lentil plants treated with 4 different concentrations (, , 1%, and ) of the herbicide fluazifop-p-butyl (FPB) were determined. The experiments were performed with light and transmission electron microscopy (TEM) and the chlorophyll content of the leaves was determined using spectrophotometric methods. | Ö. AKSOY, F. DANE Research Article Turk J Bot 35 (2011) 389-402 © TÜBİTAK doi: Ultrastructural changes in the root tip and leaf cells of Lens culinaris treated with fluazifop-p-butyl Özlem AKSOY1,*, Feruzan DANE2 1 Department of Biology, Faculty of Science and Arts, Kocaeli University, Umuttepe Campus, 41380 İzmit, Kocaeli - TURKEY 2 Department of Biology, Faculty of Science and Arts, Trakya University, Güllapoğlu Campus, Edirne - TURKEY Received: Accepted: Abstract: In this study, ultrastructural changes in root tip cells and leaf cells of lentil plants treated with 4 different concentrations (, , 1%, and ) of the herbicide fluazifop-p-butyl (FPB) were determined. The experiments were performed with light and transmission electron microscopy (TEM) and the chlorophyll content of the leaves was determined using spectrophotometric methods. The effect of FPB included alterations in cell walls, nuclei, plastids, cuticle, and epidermis. The obtained results indicate that the herbicide FPB has the ability to cause different kinds of abnormalities in various cell organelles, especially the cell walls in root tips. The effect of FPB was not limited only to the anatomy and plastid ultrastructure of the leaf, but the chlorophyll content was also affected. Key words: Lens culinaris, Leguminosae, root tip, leaf, organelles, chlorophyll Fluazifop-p-butyl ile muamele edilmiş mercimeğin kök ucu ve yaprak hücrelerindeki ince yapı değişimleri Özet: Bu çalışmada bir herbisit olan fluazifop-p-butyl’in (FPB) 4 farklı (% 0,25, % 0,5, % 1 ve % 1,5) konsantrasyonu ile muamele edilmiş mercimek kök ucu ve yaprak hücrelerindeki ince yapı değişimleri belirlendi. Deneyler ışık ve geçirgen elektron mikroskobu (TEM) ile gerçekleştirildi ve yapraklardaki klorofil içeriği spektrofotometrik yöntemle saptandı. FPB’in etkileri hücre duvarı, hücre çekirdeği, plastidler, kütikula ve epidermisdeki farklılıkları içermektedir. Elde .
