Induction of embryogenic tissue from immature zygotic embryos in Pinus nigra J.F.Arnold subsp. nigra var. caramanica (Loudon) Businsky

Embryogenic cultures (EC) of Anatolian black pine (Pinus nigra subsp. nigra var. caramanica (Loudon) Businsky) were initiated from immature pre-cotyledonary zygotic embryos sampled weekly from 16 different trees in 2 consecutive years. Douglas-fir cotyledon revised (DCR) medium supplemented with µM of 2,4-D and µM of BAP was used for initiation and maintenance of EC. | Turk J Bot 32 (2008) 179-183 © TÜB‹TAK Research Article Induction of Embryogenic Tissue from Immature Zygotic Embryos in Pinus nigra subsp. nigra var. caramanica (Loudon) Businsky 1 1,2 1 1, Zeynep ÖZKURT , Tolga YILDIRIM , Sertaç ÖNDE , Zeki KAYA * 1 Middle East Technical University, Department of Biological Sciences, 06531 Ankara - TURKEY 2 Akdeniz University, Department of Biology, 07058, Kampüs, Antalya - TURKEY Received: Accepted: Abstract: Embryogenic cultures (EC) of Anatolian black pine (Pinus nigra subsp. nigra var. caramanica (Loudon) Businsky) were initiated from immature pre-cotyledonary zygotic embryos sampled weekly from 16 different trees in 2 consecutive years. Douglas-fir cotyledon revised (DCR) medium supplemented with µM of 2,4-D and µM of BAP was used for initiation and maintenance of EC. Overall initiation frequencies of EC in the study were in 2004 and in 2005; tree values ranged from to . Overall, and of the initial explants were converted into established cell lines in 2004 and 2005, respectively. Key Words: Pinus nigra subsp. nigra var. caramanica, somatic embryogenesis, initiation, embryogenic cultures Pinus nigra subsp. nigra var. caramanica (Loudon) Businsky Olgunlaflmam›fl Zigotik Embriyolar›ndan Embriyogenik Dokular›n ‹ndüklemesi Özet: Karaçam embriyogenik dokular› (ED), birbirini takip eden iki y›l boyunca haftal›k olarak 16 farkl› a¤açtan toplanan olgunlaflmam›fl henüz kotiledon geliflimi göstermeyen zigotik embriyolardan elde edildi. ED’lerin indüklemesi ve sonras›nda ço¤alt›lmas› için, 13,6 µM 2,4-D ve 2,2 µM BAP ilave edilmifl Douglas-fir cotyledon revised (DCR) besiyeri kullan›ld›. ED genel indükleme frekanslar› 2004 y›l›nda %0,92, 2005 y›l›nda ise %1,96 olarak gerçekleflti; farkl› a¤açlar için de¤erler s›f›r ile %7,32 aras›nda de¤iflti. ED indüklemesinde kullan›lan explantlar, 2004 ve 2005 y›llar›nda yap›lan örneklemelerde s›ras›yla .