The dendrogram obtained from UPGMA analysis revealed grouping of populations with respect to their forest division, except with Kullu forest division. The existence variation among 28 populations based on percentage of polymorphic bands (PPB) was proved to be coupled with geographical altitude (r = ). | Research Article Turk J Bot 33 (2009) 1-12 © TÜBİTAK doi: Congruence of RAPD and ISSR Markers for Evaluation of Genomic Relationship Among 28 Populations of Podophyllum hexandrum Royle from Himachal Pradesh, India Md. Afroz ALAM, Pradeep Kumar NAİK*, Gyan Prakash MISHRA Department of Bioinformatics and Biotechnology, Jaypee University of Information Technology, Waknaghat, Solan 173215, Himachal Pradesh - INDIA Received: Accepted: Abstract: Twenty eight populations of Podophyllum hexandrum Royle were selected to study genetic relationship using RAPD and ISSR markers from north-western Himalayas, Himachal Pradesh, India. Nineteen RAPD primers and 11 ISSR primers amplified a total of 131 and 68 scorable bands, of which and , respectively, were polymorphic. The mean coefficient of gene differentiation (Gst) was and , indicating that Nei’s gene diversity of and reside in all the populations. Estimated value of gene flow for RAPD (Nm = ), for ISSR (Nm = ) individually, and the combination of RAPD+ISSR (Nm = ) markers indicated that there was limited gene flow among the populations. The dendrogram obtained from UPGMA analysis revealed grouping of populations with respect to their forest division, except with Kullu forest division. The existence variation among 28 populations based on percentage of polymorphic bands (PPB) was proved to be coupled with geographical altitude (r = ). The genetic similarity matrices generated by ISSR and RAPD markers were highly correlated (r = at P = ), showed similar estimation between the 2 systems. Both markers were equally useful in providing some understanding about the genetic relationship of different populations in Podophyllum L. Key Words: Podophyllum hexandrum, RAPD, ISSR, RAPD+ISSR, Genetic Diversity Introduction The Himalayan region is home to numerous highly valued medicinal plants including Podophyllum .
