An improved protocol for the isolation of DNA from herbarium material of some lichen specimens is described. The isolated DNA is suitable for PCR reactions for DNA sequence analysis. The hexadecyltrimethylammonium bromide (CTAB) based protocol defined in this study provides a number of advantages, mainly speed and reliability. | Turk J Bot 30 (2006) 449-453 © TÜB‹TAK Research Article Isolation of DNA for Sequence Analysis from Herbarium Material of Some Lichen Specimens Sümer ARAS1,*, Demet CANSARAN2 1 Biotechnology Section, Department of Biology, Faculty of Science, University of Ankara, 06100 - TURKEY 2 Botany Section, Department of Biology, Faculty of Science, University of Ankara, 06100 - TURKEY Received: Accepted: Abstract: An improved protocol for the isolation of DNA from herbarium material of some lichen specimens is described. The isolated DNA is suitable for PCR reactions for DNA sequence analysis. The hexadecyltrimethylammonium bromide (CTAB) based protocol defined in this study provides a number of advantages, mainly speed and reliability. In addition, different DNA extraction protocols were examined to determine the yield of DNA from the thallus of lichen specimens. The methods examined include a CTAB based protocol and a sodium dodecyl sulphate (SDS) based protocol. Although these procedures yielded DNA of suitable purity for PCR analysis, the improved protocol yielded DNA of higher quality. The recovery of DNA with an average yield of 25 mg/g of herbarium material was possible with this procedure. Key Words: DNA isolation, herbarium material, lichen, sequence analysis Baz› Likenlere ait Herbaryum Örneklerinde Dizi Analizi için DNA ‹zolasyonu Özet: Baz› likenlerin herbaryum örnekleri için gelifltirilmifl bir DNA izolasyon yöntemi belirlenmifltir. Bu yöntemle izole edilmifl DNA, DNA dizi analizinin PCR reaksiyonlar› için uygundur. Bu çal›flmada tan›mlanm›fl olan hekzadesiltrimetilamonyum bromür (CTAB)’e dayal› bu protokol baflta h›z ve güvenilirlik olmak üzere birçok avantaj sa¤lamaktad›r. Buna ek olarak liken türlerinin talluslar›ndan elde edilen DNA’y› belirlemek için farkl› DNA ekstraksiyon yöntemleri kullan›lm›flt›r. Denenen metodlar hekzadesiltrimetilamonyum bromür (CTAB)’e ve sodyum dodesil sülfat (SDS)’a ba¤l› birer yöntemi kapsamaktad›r. Bu .
