The present study aimed to test the utility of RAPD (randomly amplified polymorphic DNA) and cpSSR (simple sequence repeats) markers for in situ gene conservation programs for fir species, as well as for determining the genetic similarities between the Abies nordmanniana Spach species complex (A. nordmanniana, A. bornmuelleriana Matff., A. equi-trojani (Asch. & Sint. ex Boiss) Mattf.) and between populations of A. equi-trojani, which is a narrow-endemic to Turkey. | Turk J Bot 32 (2008) 1-10 © TÜB‹TAK Research Article Genetic Differentiation of Abies equi-trojani (Asch. & Sint. ex Boiss) Mattf. Populations from Kazda¤›, Turkey and the Genetic Relationship between Turkish Firs belonging to the Abies nordmanniana Spach Complex 1 2 2,3 Zeki KAYA *, A. SKAGGS , David Brian NEALE 1 Department of Biological Sciences, Middle East Technical University, 06531 Ankara - TURKEY 2 Institute of Forest Genetics, USDA Forest Service, Pacific Southwest Research Station, 2480 Carson Rd., Placerville CA 95667 USA 3 Department of Plant Sciences , University of California at Davis, One Shields Avenue, Davis, CA 95616 USA Received: Accepted: Abstract: The present study aimed to test the utility of RAPD (randomly amplified polymorphic DNA) and cpSSR (simple sequence repeats) markers for in situ gene conservation programs for fir species, as well as for determining the genetic similarities between the Abies nordmanniana Spach species complex (A. nordmanniana, A. bornmuelleriana Matff., A. equi-trojani (Asch. & Sint. ex Boiss) Mattf.) and between populations of A. equi-trojani, which is a narrow-endemic to Turkey. For this purpose, DNA was extracted and pooled from 15 seed megagametophytes (megs) of the Ortaköy population of A. nordmanniana and the Muratdere population of A. bornmuelleriana species, and from two 7-meg subsamples each from of the Kazda¤› and Çan populations of A. equi-trojani. Template DNA was screened with the DNA markers to reveal the amount of genetic variation in each species. It appeared that template DNA pooling for screening the fir populations with RAPD or cp-SSR markers could be effectively used to speed up gene conservation and taxonomic studies. It is suggested that DNA pooling for template DNA in the PCR (polymerase chain reaction) mixture should be limited to 7 megs, but at least 5 replications that sample different sets of families each time. Based on genetic similarity and distance .
