This study aims at developing a regional algorithm to quantify chlorophyll-a concentration (Chla) in the Thac Ba Reservoir surface-water using Landsat 8 imagery basing on in-situ data of Chla and above-water reflectance taken in both dry and rainy seasons 2018. In situ datasets obtained from 30 water sampling sites show a strong correlation (R2=) with the reflectance ratio of two Landsat 8 (L8) bands, the green band (band 3: B3) versus the red band (band 4: B4), B3 / B4, by an exponential equation.