In in-vitro-fertilization, human embryos have been cultured in incubator which are closely monitored level of components including temperature, humidity and especially level of CO2 and O2 concentration. | Journal of military pharmaco-medicine no5-2018 CLINICAL EFFECTS OF EMBRYOS CULTURE IN LOW OXYGEN CONCENTRATION Nguyen Thi Minh*; Nguyen Viet Tien*; Quan Hoang Lam** SUMMARY In in-vitro-fertilization, human embryos have been cultured in incubator which are closely monitored level of components including temperature, humidity and especially level of CO 2 and O2 concentration. Some centers are using 20% O 2 while others apply only 5% O2 and with this low level of O2, IVF outcome is promising. However, there is no evidence about the optimized level of O2 concentration in human embryos culture. Objectives: To compare pregnancy rate, live birth rate between 2 groups is 5% O2 and 20% O2. Methods: This is a randomized clinical trial study among 172 patients who were divided into 2 groups: embryos cultured in 5% and 20% oxygen concentration. Before culture, using FSH to stimulate ovaries. Oocytes retrieval was done after hCG injection 36 hours. Fertilization was made by conventional IVF or ICSI. On day 3, 3 - 5 top quality embryos were selected to culture to day 5 in both groups. Residual embryos were frozen by vitrification method. Results: No differences in characteristics of ovarian reserve, ovarian stimulation and embryos transfer in both groups. Clinical pregnancy rate in 5% oxygen group was , significantly higher than in 20% oxygen group. Live birth rate in 5% oxygen group was , higher than in 20% oxygen group but no significantly. Conclusion: Clinical pregnancy rate on day 5 in 5% oxygen group was higher than that in 20% oxygen group. Live birth rate in 5% oxygen group was higher than in 20% oxygen group but no significantly. * Key words: Pregnancy rate; Live birth rate; Low oxygen concentration in IVF. INTRODUCTION The technique of in-vitro fertilization (IVF) has been increasingly advanced in order to improve the success rate. Many studies in the world state that blastocyst transfer is close to physiology and selected embryos with good .
