Helicteres hirsuta Lour., particularly its stems and leaves have been used in Vietnamese traditional medicine to cure furuncles, dysentery, measles and flu. In this study, we examined biological activities of 5 fractions from methanol extract of H. hirsuta roots. The results showed that ethyl acetate fraction has the highest content of phenolic and flavonoid compounds ( ± mg GAE, gallic acid equivalents/g dry weight and ± mg QE, quercetin equivalents/g dry weight, respectively). | Second metabolites and antioxidant, antimicrobial, anticancer activities of Helicteres hirsuta root extract