Trong những năm gần đây, phương pháp dựa trên metagenomics để phân lập các hợp chất mới từ môi trường biển đang ngày càng được chú ý. Từ thư viện metagenome, bằng phương pháp tin sinh học có thể sàng lọc các gen có hoạt tính sinh học mới từ vi sinh vật không thông qua nuôi cấy. Đây thực sự là bước đột phá trong nghiên cứu và ứng dụng của công nghệ sinh học. | Sàng lọc gen mã hóa protein ức chế protease từ metagenomics của vi sinh vật liên kết với hải miên biển Quảng Trị, Việt Nam TAP CHI SINH HOC 2019, 41(2): 49–60 DOI: SCREENING GENES ENCODING PROTEIN PROTEASE INHIBITOR FROM METAGENOME OF SPONGE-ASSOCIATED MICROORGANISMS IN QUANG TRI SEA, VIETNAM Tran Thi Hong1,2,*, Pham Viet Cuong1, Nguyen Thi Kim Cuc3 1 Mientrung Institute for Scientific Research, VAST, Vietnam 2 Graduate University of Science and Technology, VAST, Vietnam 3 Institute of Marine Biochemistry, VAST, Vietnam Received 13 March 2019, accepted 5 May 2019 ABSTRACT Using metagenomics-based method to isolate new compounds from the marine environment are getting more and more attention in recent years. Based on metagenome library, bioinformatics methods is a powerful tool for screening genes with new biological activities from uncultured microorganisms and become a breakthrough in research and application of biotechnology. In this study we selected and used the samples DNA QT2 which had high DNA content and purity from a total of 6 DNA samples of sponge-associated microorganisms collected in Quang Tri sea (Vietnam) for metagenomic sequencing (DNA concentration is ng, A260/A280 value is ). 16S rRNA metagenomic sequencing data of QT2 produced 44,117,722 reads, which were assembled into 120,236 contigs. ORF prediction using Prodigal produced 386,416 ORFs. Functional annotation was conducted based on 7 different databases (NR, COG, CAZy, Swissprot, GO, KEGG, Pfam), and there are 266,553 genes were annotated using Swiss-Prot. In addition, based on the obtained metagenomic data, 50 complete genes encoding protease inhibitor proteins were revealed and among them, 28 genes encoding protein (> 50%) belonged to the serine protease inhibitor family, and 22 genes genes encoding belonged to the Inter-alpha- trypsin inhibitor group. NCBI BLAST screening .
