HPLC-PDA method was used to determine two basic dyes, Auramine O and Rhodamine B in food samples. The limits of detection and quantification of Auramine O (AO) and Rhodamine B (RB) were both mg/L and mg/L, respectively. The recoveries of AO and RB in matrices ranged from to with the concentration µg/g, µg/g and µg/g. | Development and validation of HPLC-PDA method for the simultaneous determination of Auramine O and Rhodamine B in foodstuffs