Molecular cloning of stress-induced genes of maize (Zea mays L.) using the PCR-select cDNA subtraction technique

Environmental abiotic stresses, such as drought, high-salinity and low temperature, severely impair plant growth and development and limit crop productivity. In order to survive and adapt to these stresses, plants must induce various physiological, bichemical and molecular changes, including the adaptation of the photosynthetic apparatus, changing in the membrane lipid, the activation of calcium influxes and Ca2+-dependent protein kinase cascades, the accumulation of proline, glycine betaine, soluble sugars and increasing the levels of antioxidants. All these changes are accompanied by notable increases or decreases in the transcript level of specific genes. Hence, transcriptional control of stressregulated genes is a crucial part of plant responses to abiotic stresses; a further characterization of such gene transcripts in plants may help us to understand the molecular basis of the plant response to abiotic stresses and to identify new targets for manipulating biochemical, physiological and developmental processes in plants. | Molecular cloning of stress-induced genes of maize (Zea mays L.) using the PCR-select cDNA subtraction technique

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