Khảo sát hoạt tính chống oxy hóa, chống viêm và hoạt tính bảo vệ gan trên chuột tổn thương gan bằng carbon tetrachloride của cao chiết lá Trang to (Ixora duffii)

Cây Trang to (Ixora duffii) thuộc chi Ixora họ Rubiaceae. Hoạt tính chống oxy hóa và chống viêm in vitro của cao methanol lá I. duffii được khảo sát bằng phương pháp phosphomolybdenum và phương pháp ức chế sự biến tính albumin huyết thanh bò. | Khảo sát hoạt tính chống oxy hóa, chống viêm và hoạt tính bảo vệ gan trên chuột tổn thương gan bằng carbon tetrachloride của cao chiết lá Trang to (Ixora duffii) TAP CHI SINH HOC 2019, 41(1): 117–128 DOI: ANTIOXIDANT, ANTI-INFLAMMATORY AND HEPATOPROTECTIVE ACTIVITIES ON CARBON TETRACHLORIDE - INDUCED HEPATIC DAMAGE IN MICE OF Ixora duffii LEAF EXTRACT Phan Kim Dinh, Nguyen Trong Tuan, Dai Thi Xuan Trang* College of Natural Sciences, Can Tho University Received 9 July 2018, accepted 11 February 2019 ABSTRACT The in vitro total antioxidant and anti-inflammatory capacity of the methanol extract of Ixora duffii leaf (―Trang to‖ in Vietnamese) was investigated using the phosphomolypdenum method and the bovine serum albumin denaturation test, respectively. The results showed that the methanol extract of I. duffii leaf possessed relatively high antioxidant activity (OD0,5 = ± μg/mL) compared to that of the standard, trolox (OD0,5= ± μg/mL). The anti- inflammatory effect using bovine serum albumin denaturation test showed that the activity of the methanol extract of I. duffii leaf (EC50= ± μg/mL) was comparable to that of the reference drug, diclofenac sodium (EC50= ± µg/mL). To evaluate the hepatoprotective activity of the methanol extract of I. duffii leaf, the liver damage was induced in mice given with carbon tetrachloride (CCl4) mixed in olive oil (1:4) at a dose of mL/kg body weight/day. As a control, the standard hepatoprotective agent, silymarin, was used at the dose of 16 mg/kg body weight for 4 consecutive weeks. I. duffii leaf extract was given at three different doses 100, 200 and 400 mg/kg body weight. The results showed the effective dose-dependent reduction of transaminase enzyme (ALT and AST) levels in sera. In addition, I. duffii leaf extract also improved the oxidative stress status of the liver through .