In this study, the fatty acid, tocopherol and tocotrienol composition in the seed oil of Cannabis sativa L., which is traded under the common name hemp seed oil, were determined by using GLC and HPLC techniques. While α- linolenic, linoleic, oleic and palmitic acid were the main fatty acid components, γ – linolenic (18:3 n-6) and stearidonic acid (18:4 n-3) were found as unusual minor fatty acids in the seed oil. γ – linolenic acid is an important fatty acid used both as a healthy nutrient and as a therapeutic agent. | A chemotaxonomic approach to the fatty acid and tocochromanol content of Cannabis sativa L. (Cannabaceae)