The aim of this study was to assess the predictive values of AFC, AMH and FSH in predicting high ovarian response during in - vitro fertilization (IVF). We recruited 600 IVF patients who were receiving GnRH antagonist therapy and recombinant FSH for ovarian stimulation. High ovarian response during IVF was defined as > 15 oocytes retrieved. AMH, FSH and AFC levels were assessed on cycle day 2. We found that the AMH threshold value for high ovarian response was ng/ml with a sensitivity of 73% and a specificity of 61%. The AFC threshold value for high ovarian response was , with a sensitivity of and a specificity of 52%. The FSH threshold value for high ovarian response was (IU/L) with a sensitivity of and a specificity of . The area under the curve (AUC) of AMH, AFC and FSH were 71%, 65%, , respectively. Conclusions: AMH was the best marker for predicting high ovarian response during IVF, followed by AFC and FSH. | Comparing amh, AFC and FSH for predicting high ovarian response in women undergoing antagonist protocol