The objective of this study was to target rutin, in a more solubilized form, to the colon aiming at treatment of colon carcinoma. pH sensitive nanospheres were prepared by the nanoprecipitation technique employing Eudragit S100. Different drug: polymer ratios as well as different concentrations of the stabilizer Poloxamer-188 were used. | Formulation and evaluation of pH-sensitive rutin nanospheres against colon carcinoma using HCT-116 cell line