The importance of hands in the transmission of hospital infections has been well demonstrated and hand hygiene reduces the prevalence of hospital acquired infections. The study was designed to evaluate the knowledge, attitude, practices and satisfaction of the available facilities among Postgraduates and CRRIs and to identify the gaps in hand hygiene practices and to implement an Institutional program on hand hygiene policy. A self-administered questionnaire was used. The study was conducted between September 2018 and November 2018. A total of 275 participants (148 Postgraduates and 127 CRRIs) participated in the study. Data was analyzed using Microsoft excel 2010 software. Pearson chi-square test was used to check for statistically significant differences. A p-value of less than was considered significant. Majority had moderate knowledge () whereas postgraduates had better knowledge than CRRIs. The study population had moderate attitude () and practices (44%). Postgraduates had good attitude and practices than the CRRIs. suggested improvement of the available facilities. A need for hand hygiene training program and multifaceted interventional behavioral program particularly for the medical students to improve the compliance. | Hand hygiene knowledge, attitude and practices among postgraduates and Crris in a Tertiary Care Hospital, Chennai, India