The present study is an attempt to differentiate tomato hybrids from its parental lines using stomatal characters like stomatal density, length and width of guard cells on both the adaxial and abaxial surfaces and to assess the genetic variation for these traits. Significant differences were found for stomata characters like stomata density, length, width and area of stomata on both adaxial and abaxial surfaces of cotyledonary leaf. Hybrids recorded significantly higher stomata densities than one or both their parental lines. The differences were more conspicuous on the abaxial surface than adaxial surface. The present study was an attempt to differentiate hybrid from its parental lines and the hybrid had clear distinction from one of its parental line. | Genetic variation of stomatal traits in four tomato hybrids and their parental lines