The objective of our study was to identify the associations between the tests that used in ovarian reserve assessment: Anti-Mullerian hormone (AMH), follicle stimulating hormone (FSH) and antral follicle count (AFC) and to distinguish the most reliable markers for ovarian reserve in order to select an adequate strategy for the initial stages of infertility treatment and identify the effect of vitamin D on the ovarian reserve and its correlation with AMH. Anti-mullerian hormone (AMH) is expressed only in the gonads. In female it’s secreted by adult granulosa cells of the ovary. The levels of AMH reflect the number of preantral follicles and thus as a marker of oocyte pool which is a germinal reserve of the ovary for reproduction. Vitamin D is a fat-soluble vitamin that belongs to the family of steroid hormones. It has a biologically plausible role in female reproduction. In this paper AMH, FSH and Vitamin D was determined by enzyme linked immunosorbent assay in: 60 infertile women with PCOS (cases) undergoing IVF and 30 healthy women had one child at least (control). The antral follicle count (AFC) was recorded for each female in case group. The AMH and 25(OH) D levels in cases were lower than that of control, while FSH level in cases was higher than control group. The AMH in cases was significantly decreased with increasing age. | The associations of serum AMH, vitamin D, FSH and AFC in different age groups of infertile women
