In maize, zeins are the main protein components of seed stores. It is the major determinants of nutritional imbalance when utilized as the sole food source. Zeins having four subfamilies (α, β, γ, and δ). Among these, α zeins are the major prolamin subunits in maize. Opaque-2 (o2) is a natural recessive mutation that is exploited for breeding varieties. However, it possessed some adverse pleiotropic effect so, the combination ofopaque-2 allele with its genetic modifiers composed to breed QPM genotypes that having a hard kernel with a high content of lysine and tryptophan. However, the biochemical analysis of lysine and tryptophan content is expensive as well as it is , conventional breeding alone is inefficacious for the nutritional enrichment of maize. By using RNAi, it is proved that down regulation of 22kDa α zeins than the 19kDa α component is the biochemical basis of QPM phenotype. Whereas, marker-assisted selection (MAS) provide excellent opportunities for the conversion of elite normal in breds to homozygous recessive o2forms by using opaque-2 gene-specific markers.
