Semen cryopreservation is a widely used technique across the world for conservation and proliferation of genetically superior germplasm through artificial insemination. Artificial insemination (AI) is the most conclusive tool to increase genetic improvement, control of venereal diseases and improve herd performance and productivity. For achieving the mission, successful and effective cryopreservation of semen is essential. In recent years, increased demand of animal products due to very rapid growth of human population inspired the investigators to increase per animal milk production using various techniques of Biotechnology. The process of semen cryopreservation is stressful and around 40%- 50% of sperm lose their motility and viability due to loss of structural and functional capabilities. One of the factors which affect post thaw survival of sperm is the freezing rate. In buffalo, poor fertility rate with cryopreserved semen is main obstacle in its proliferation. It is need of the time to spread the knowledge of various factors and components such as use of suitable extenders, additives, cryoprotectants, freezing and thawing protocols contributing in cryopreservation of semen to improve the fertility rate of buffalo. | Cryopreservation of buffalo bull semen- restriction and expectation: A review
