The development of a protocol for biosynthesizing nanomaterial in an eco-friendly manner is a major concern in the field of microbial nanotechnology. In this study, microbial synthesis of silver nanoparticles (AgNPs) with a high level of bionanocatalytic activity was accomplished utilizing cell extracts of Penicillium digitatum FCMR-728 as the agents for reducing, capping and stabilizing. The presence of AgNPs was confirmed by an indication of a surface plasm on resonance band via UV–vis spectrum at 550 nm. Evidence of fairly uniformly spherical AgNPs being synthesized was shown by transmission electron microscopy images. The sizes of the nanoparticles increased with reaction time by an average of 5 nm to 30 nm. The formation of nanocrystalline silver particles was verified by X-ray diffraction analysis. The presence of functional groups on the surface of biosynthesized AgNPs, such as C=O, O―H, N―H, C―O―C C―H and C―OH, which play role in the AgNPs stability, was shown by Fourier transform infrared spectra. Biogenic AgNPs were utilized as bionanocatalysts for nanoreduction of 4-nitrophenol compound, where the linear correlation with AgNPs concentration of the reaction rate constant led to the rate increasing from /min to /min, via the AgNPs amounts increasing from ×10–6 to ×10–6 mM. The synthesized AgNPs also showed a very high degree (×105 /min/M) of normalized bionanocatalytic activity. | Microbial synthesis of sliver nanoparticles by Penicillium Digitatum FCMR-728 and their bionanocatalytic reduction of 4-Nitrophenol and antibacterial activity
