A study was conducted in vitro to control Colletotrichum dematium causing anthracnose disease of groundnut with Trichoderma harzianum and botanicals. Five botanicals viz., Datura leaf extract, Tulsi leaf extract, Garlic bulb extract, Neem oil and Eucalyptus oil at the rate of 5% were evaluated for their efficacy against the radial colony growth of C. dematium. The complete inhibition was obtained in Eucalyptus oil (100%) followed by T. harzianum (), datura leaf extract (), tulsi leaf extract (), neem oil () and garlic bulb extract (). In the present study different culture media viz., malt extract agar, Czapek dox agar, corn meal agar, Martin’s rose Bengal agar and oat meal agar were used for the study of different cultural characters of Colletotrichum dematium. | In vitro evaluation of Trichoderma Harzianum and botanicals on the radial growth of Colletotrichum dematium causing anthracnose disease of groundnut (Arachis hypogaea L.)