16S rRNA gene sequences are most commonly used for determination or studying the bacterial phylogeny and taxonomy because its present in almost all bacterial population. 16S rRNA gene sequence is remaining conversed during evolution so that 16S rRNA gene identification method is widely used for identification of bacterial diversity. In this study, bacterial population was isolated from soil sample for copper nanoparticles synthesis and maximum copper synthesizing bacteria further used for characterization. Bacteria firstly characterized by using microscopic and biochemical characters and then confirmed by using 16S rRNA gene technology. In this experiment, we have used blastn, Cluster W, software to find homology and phylogenetic analysis to identification of bacterial isolate. We have identified Stenotrophomonas maltophilia strain with a noble trait such as to synthesizing copper nanoparticles. | In silico analysis of copper nanoparticles synthesizing bacteria contributing towards big data bank