The present investigation entitled as “Effect of hardening media and different cross combinations on physiological parameters of in vitro cultured citrus plantlets” was carried out in the Division of Fruits and Horticultural Technology, ICAR-IARI, New Delhi. The experiment consisted of embryo rescued plantlets from cross pollination (control pollination) and open pollination (uncontrolled pollination) and two hardening media HM1(Coco peat: Perlite: Vermiculite, 1:1:1) and HM2 (Coco peat: Perlite: Vermiculite, 2:1:1). The results revealed that the progenies of ALC-2 x Kagzi Kalan had highest values of leaf gas exchange traits like internal CO2 concentration (Ci), transpiration rate (E), stomatal conductance (gs) and photosynthesis rate (A) which was followed by progenies of PusaUdit x KagziKalan. However, it was least in progenies of Troyer citrange (Open pollinated). Considering the hardening media, there was a general trend of the higher leaf gas exchange in the HM1media (Coco peat: Perlite: Vermiculite, 1:1:1) compare to HM2 media (Coco peat: Perlite: Vermiculite, 2:1:1). | Effect of hardening media and different cross combinations on leaf gas exchange parameters of in vitro cultured citrus plantlets
