Methicillin resistant Staphylococcus aureus (MRSA) is one of the most important etiology of community and hospital acquired infections. With an increasing incidence of Methicillin resistant Staphylococcus aureus (MRSA), the aim of the present study was to determine the prevalence of Methicillin resistant Staphylococcus aureus strains and their antibiotic susceptibility pattern in a tertiary care Hospital between July 2017 and July 2018. In this prospective cross sectional study, 100 Staphylococcus aureus were isolated and identified conventionally from various clinical specimens collected from different departments of the hospital. Subsequently, antimicrobial susceptibility test was performed by Kirby Bauer disc diffusion method as per Clinical and Laboratory Standards Institute (CLSI) guidelines. Among the 100 S. aureus isolates, MRSA was found to be 54% by cefoxitin (30 μg) disk diffusion method out of which maximum numbers were isolated from the age group of more than 60 years . 14 isolates () predominantly in males. Among the 54% of MRSA isolated, maximum were from pus, from blood, each from sputum, urine and drain and each from Ascitic fluid and tracheal aspirate. | Prevalence and antibiotic resistance pattern of Methicillin resistant staphylococcus aureus isolates in a tertiary care hospital