Three natural compounds including betulin (1), betulinic acid (2) and lupeolactone (3) were isolated from the dichloromethane extract of Helicteres hirsuta Lour. for the first time. Their structures have been elucidated by modern spectroscopic methods as IR, 1H-NMR, 13C-NMR, DEPT, HSQC, HMBC, MS and by comparison with those of previously reported data. | Derivatives of triterpene isolated from the dichloromethane extract of helicteres hirsuta lour AGU International Journal of Sciences – 2019, Vol. 7 (4), 12 – 19 DERIVATIVES OF TRITERPENE ISOLATED FROM THE DICHLOROMETHANE EXTRACT OF HELICTERES HIRSUTA LOUR Nguyen Van Ky1, Nguyen Huu Duyen1, Le Thanh Phuoc1 1 College of Natural Sciences, Can Tho University, Vietnam Information: ABSTRACT Received: 07/08/2018 Three natural compounds including betulin (1), betulinic acid (2) and Accepted: 03/01/2019 lupeolactone (3) were isolated from the dichloromethane extract of Published: 11/2019 Helicteres hirsuta Lour. for the first time. Their structures have been Keywords: elucidated by modern spectroscopic methods as IR, 1H-NMR, 13C-NMR, Betulin, betulinic acid, DEPT, HSQC, HMBC, MS and by comparison with those of previously lupeolactone, Helicteres reported data. hirsuta Lour 1. INTRODUCTION Lour. was resistant to some experimental Helicteres hirsuta Lour. was introduced in the cancer cells (Chin YW et al., 2006). previous paper of the research group (Duyen, With the above-mentioned biological activities, . and Phuoc, ., 2016; Ky, ., et al., Helicteres hirsuta Lour. is potentially useful in 2018). Regarding the bioactivities, the results of cancer treatment. Therefore, in this paper, the cytotoxic activity on Hep-G2 cell line of four research team continues to publish results of a extracts including petroleum ether, chemical composition survey to contribute to a dichloromethane, ethyl acetate and methanol, more scientific explanation of the showed that two extracts of petroleum ether and pharmaceutical application of this herb. dichloromethane gave positive test results with 2. MATERIALS AND METHODS the IC50 values being µg/mL and Material: The specimens were stems, leaves µg/mL, respectively. Regarding the chemical and flowers of Helicteres hirsuta Lour. constituents, the research team isolated six .
