In this investigation two different methods using aqueous solutions of colchicine were used to treat F1 tillers from various Triticum durum x Aegilops tauschii crosses in order to artificially induce chromosome doubling. Treatment of crown root region (uproot) was found effective compared to in-situ (tip) method using treatment of apical meristems of F1 tillers at 2-3 tiller stage. The aqueous solution of colchicine was administered at two concentrations viz., and in both the methods. The colchicine solution was found more effective as more doubled seed was obtained. | Comparison between conventional and in-situ chromosome doubling method in Triticum Durum X Aegilops Tauschii crosses
