This prospective study was done to determine correlation between urine and/or stone cultures with postoperative sepsis in patients treated for renal and ureteral calculi. This prospective study was carried out in Department of Microbiology, Government medical college Kota between July 2015 and June 2016. The incidence of renal stone was more in male (N 70) (70%) as compared with female (N 30) (30%) ., in the ratio of 2:1. Urine culture was positive more in females ( in female vs. in male approx. ratio 2:1), however infected stone rates are almost similar in male and female ( in male vs. in female). Out of 100 patients 43 cases showed infection in preoperative urine culture, while rests 57 were sterile. Similarly 64 patients were positive for stone culture and rest 36 were sterile. 28 patients had both stone and urine culture positive. Most common bacteria isolated in urine culture were E. coli, Klebsiella positive urine culture) cases. Post operatively 35% (N 35) patient show signs of SIRS. Out of 35 patients who had sepsis 30 patients were positive for stone culture while 20 patients were positive for urine culture. | A study on positive stone culture and its association with rate of sepsis after urological procedures