Trichoderma harzianum parasitism on Meloidogyne incognita eggs and juveniles was examined in-vitro under Assam condition. M. incognita egg masses, their derived eggs and second-stage juveniles (J2) were parasitized by T. harzianum. The conidia of the T. harzianum were found inside of the eggs and attached to the J2s with the gelatinous matrix. The eggs were penetrated and parasitized by the hyphae of T. harzianum, while eggs containing juveniles were also parasitised by T. harzianum. Further, isolate T. harzainum was used for to know the bio-efficacy against M. incognita infected on okra under pot condition. For this T. harzianum was applied either as a seed treatment and/or soil application or both. Carbosulfan as a seed treatment and carbofuran as soil application was applied as chemical checks both either singly or in combination. The results showed that either T. harzianum or the chemicals (Carbofuran and Carbosulfan) when applied together as a seed treatment and soil application, improved plant growth parameters of okra and reduced the nematode multiplication as compared to when they were applied either as a seed treatment or soil application. Application of chemicals either as a seed treatment or soil application emerged as the most effective treatment as compared to the T. harzianum. However, in respect of T. harzianum when applied together as a seed treatment and soil application showed significantly better results in an improving the plant growth parameters and reduction in the nematode multiplication as compared to the treatments with carbosulfan as a seed treatment and carbofuran as soil application alone. | Biological control of Meloidogyne Incognita by Trichoderma harzianum
