This study focused its investigation on the use of the essential oil of a culinary and medicinal plant commonly used in Cameroon, Curcuma longa also called red ginger for the protection of white yam (Discorea rotoundata) during storage. The oil was obtained by hydro distillation using Clevenger apparatus with a yield of , the chemical composition analysis revealed a predominance of oxygenated sesquiterpens (OST ) with as major compound α-bisabolol (), E-β-santalol (), β-cymene (). Fungi isolated from apparently healthy yam tubers were identified as Rhizopus stolonifer and Penicillium sp., In vitro and in situ oil activity was evaluated. The oil completely inhibited the growth of in vitro pathogens at 1150 and 6000 ppm respectively for Rhizopus stolonifer and Penicillium sp., the oil being more active on Rhizopus stolonifer. In situ tests were carried out only with this fungus. The essential oil of Curcuma longa has significantly reduced the rot caused by this pathogen, a reduction of and was obtained for the preventive and curative tests respectively. No significant difference in activity was observed in both tests. However, there is a significant difference (P ≤ ) in activity between the essential oil and the reference fungicide at the recommended concentration. As concerning the activity of this essence, the red ginger essential oil could be used as a biofungicide for the protection of yam tubers preserved against R. stolonifer, the most formidable fungus of rot white yam in Cameroon. | Chemical composition of the essential oil of Curcuma longa and evaluation of the antifungal activity on Rhizopus stolonifer and Penicillium sp. responsible fungi for post-harvest rot of Dioscorea rotoundata in cameroon