Response surface methodology (RSM) is a combination of statistical and mathematical techniques used to create the model and to analyze a response influenced by several factors. The present research was carried out to enhance bacteriocin production by the Lactic acid bacteria Lactobacillus gasseri NBL 18 isolated in our lab from infant fecal samples. The influence of physical parameters viz. temperature (37-42°C), pH (), incubation time (6-24h) and inoculum level (1- 3%) on bacteriocin production was analyzed through RSM. Maximum bacteriocin production of X 104 AU/ml was obtained at temperature 37°C, pH , inoculum size 3% and incubation time of 24 h. Statistical analysis showed that all the four factors had significant effects on bacteriocin production. RSM proved to be a powerful tool in the optimization of bacteriocin production by L. gasseri NBL 18. | Optimization of bacteriocin production from Lactobacillus Gasseri NBL 18 through response surface methodology
