Urinary tract infections (UTI) are the second most common infections encountered in clinical practice and is associated with a high rate of morbidity and economic burden. Knowledge of the local antibiotic resistance patterns will help in providing empirical therapy and helps in prevention of resistance. This was a retrospective study conducted from January 2017 to December 2018 at a medical college in South India. Samples received included mid-stream clean catch urine, Catheterized urine, suprapubic aspirate. Urine specimen was collected in a sterile, wide mouth, leak proof, labelled container. Urine was processed immediately within one hour without delay. Samples were processed and isolates were identified as per standard methods. Antibiotic sensitivity testing was done on Mueller Hinton agar by Kirby–Bauer disc diffusion method and CLSI guidelines. Most UTI cases were seen in females and in the age group of 31-40 years. The most common organism isolated was E. coli (), followed by Klebsiella species () and Pseudomonas aeruginosa (). The most common Gram-positive organism was Staphylococcus aureus (). | Bacteriological profile of urinary tract infection at a Tertiary care Hospital in Kalaburagi, India