Effective invertase enzyme production was achieved with orange peel as carbon source compared to all other tested also residues. Among different nitrogen sources, yeast extract supported maximum enzyme production. Various fermentation parameters (pH of the medium, incubation temperature, time, volume in addition to carbon and nitrogen sources) also influenced the rate of invertase production. Maximum enzyme production of 55 units was observed in the medium of pH 4 containing 2% of orange peel having particle size of mm containing 1% of sucrose and 1% yeast extract in 96 hours of incubation. | Orange peel as novel substrate for enhanced Invertase production by A. Niger in solid state fermentation