Nano-fertilizer technology is designed to deliver nutrients in a regulated pattern in correspondence with the crop demand thereby nutrient use efficiency can be improved without associated ill-effects. The present investigation was conducted to study the effect of soil and foliar application of organic nano NPK formulations on microbial load (bacteria, fungi and fungi) and their role in improving the enzymatic activities (dehydrogenase, acid phosphatase, alkaline phosphatase and urease) of the experimental soil. The field experiment was carried out during the year 2017-18 at College of Agriculture, vellayani. Fertilizer samples were analysed and particle size of granular organic nano NPK and liquid organic nano NPK were nm and nm respectively. Zeta potential of the organic nano NPK formulations was mV means the organic nano NPK formulations was considered to be highly stable. The soil samples were collected at the final harvest of the crop for calculating the microbial load and enzymatic activities of the soil. From the result it was indicated that among the different treatment combinations, application of FYM (12 t ha-1 ) + Soil application of nano NPK ( kg ha-1 ) + Foliar application of nano NPK () recorded the highest bacterial count, dehydrogenase, urease and acid phosphatase content in the post harvest status of the soil. | Organic nano NPK formulations on soil microbial and enzymatic activities on post-harvest soil of Bhindi