Ghee is known as valuable natural source of food that has numerous health benefits entirely beneficial to the human population. It is one of the popular ingredients in the Indian diet and takes infinitely prevalent position in the dairy industry market. The geographical production makes curious difference in the physico-chemical properties, flavour uniqueness, sensory and storage stability parameters. Concentrating on the geographically important ghee, uthukuli ghee from region of TamilNadu is known for its extraordinary flavour. Exclusiveness in ghee production is primarily dependent on the area of production, breed of animal, feed used for the animals, temperature and other intrinsic, extrinsic factors. In this paper, physico-chemical parameters such as Reichert- Meissl (Rm) value, Polenske value, Iodine value, Saponification value (SV), Butyro-refractometer (BR) reading, Melting Point, Free fatty acid (FFA), peroxide value, Critical Temperature of Dissolution (CTD) have been determined for geographically relevant uthukuli ghee.