In the present study investigated the taxonomic identities and phylogenetic relationships of Ganoderma species isolated from different locations within Mizoram, India using a combination of morphological and molecular approaches. Six Ganoderma isolates were identified through morphology and further confirmed by molecular phylogenetic analysis using nuclear ribosomal DNA sequences, including both the internal transcribed spacers (ITS1 and ITS2) and the gene region. The 6 species of Ganoderma identified to the species level based on morphology and fungal DNA sequences were G. lingzhi, G. mastoporum, G. mizoramense, G. multipileum, G. subresinosum, and G. williamsianum.