Gladiolus is one among the most popular commercial cut flowers of commercial importance in the world market ranked forth (Bose and Yadav 1989). A survey for the incidence of wilt of gladiolus (F. oxysporum . gladioli) was carried out at 12 locations comprising five tahsils of Aurangabad district during 2016-17. The highly prone areas of wilt of gladiolus under polyhouse in Aurangabad district were Naygaon and Bhoyagaon ( each), followed by Fardapur (). The average district mean per cent incidence of wilt of gladiolus under protected condition (polyhouse) in Aurangabad was recorded per cent. | Survey of different locations for prevalence of wilt disease of Gladiolus