In the present study, anther of three varieties and their cross combinations of Brassica campestris, namely HPBS-1, KBS-3, HPKM-04-1, HPBS-1 x HPKM-04-1 and KBS-3 x HPKM-04-1 were cultured in vitro to observe their callus induction frequency. The effect of two basal media . MS and N6, two different sucrose concentrations . 3% and 4%, three combinations of growth hormones viz. HM1, HM2, HM3 and their callus induction frequency were analyzed by CPCS software. Out of all factors and their interactions, the genotype HPBS-1 performed better in N6 medium with HM2 [ mg/l 2, 4-D + mg/l NAA] and 3 per cent sucrose for callus induction frequency. This media and hormonal combination can be successfully utilized for induction of haploids and double haploids in future. | Efficient callus induction through anther culture in cultivars of Brassica Campestris var. Brown Sarson
