Molecular Biology experiments including gene cloning, restriction digestion, identification by 18S r-DNA requires purified and high quality genomic DNA. The major problems in DNA isolation from fungus are impurities such as polysaccharides, protein and RNAs which interfere in PCR reaction. In this study, proficient method for DNA extraction without using liquid nitrogen, CTAB or lysozyme was optimized. The method utilizes very few chemical compounds. The method involved crushing of fungal mycelia in lysis buffer containing SDS, incubation at 65ºC, extraction by chloroform, phenol and isoamyl alcohol and finally DNA precipitation by cold ethanol. The results showed DNA with high yield which can be utilized for PCR purposes. | Rapid and efficient procedure for genomic DNA extraction from Trichoderma spp.
