Urinary tract infection (UTI) is one of the more common medical problems. It is an inflammatory response of the urothelium to bacterial invasion that is usually associated with bacteriuria and pyuria. Common disorder is accounting for 1-3% of consultations in general medical practice. A total of 3835 urine samples collected from clinically suspected urinary tract infection were subjected to microbiological investigations. Out of 3835 urine samples processed, 1756 () samples showed significant growth. Escherichia coli () was the most common organism isolated followed by Klebsiella (). Our study showed that majority () of the positive samples belonged to 21-40 years. Sensitivity to nitrofurantoin and amikacin was good in our study when compared to other drugs. This study shows the isolation rate of various uropthogens and their sensitivity to commonly used drugs for the treatment of UTI. | Microbiological profile of urinary tract infections at a Tertiary care Hospital