Bovine mastitis is a large-scale infectious disease of the mammary gland with significant impact on the economy of milk production along with alterations in its quality. A total of 100 mastitis suspected milk samples were collected from different organized dairy farms of Bikaner city and were evaluated for isolation, identification and antibiotic sensitivity pattern of Escherichia coli. Out of 100 mastitis suspected milk samples, 24 samples were positive for Escherichia coli (). The antimicrobial resistance profile of the tested Escherichia coli isolates to 16 different antibiotics revealed that all 24 isolates were sensitive to gentamicin, tetracycline and chloramphenicol (100%) whereas, resistant to ampicillin and penicillin-G (100%). | Antibiotic resistance profile of Escherichia coli from bovine mastitic milk samples of Bikaner region