Sophorolipid (SL) is a biosurfactant belonging to the glycolipids group and was produced by harmless Candida bombicola ACTT22214 and has been widely used in many fields in our life. In order to search for appropriate condition for C. bombicola fermentation producing SL with high efficiency, this study focused on the investigation of the soy oil concentration, molasses concentration, fermentation time, pH and temperature. SL products were tested for antimicrobial activity, antioxidant, emulsifier, foaming ability. The highest content of SL was ± g/L under conditions of: soybean oil content 5%, molasses content 150 g/L, fermentation time 7 days, pH = 5, 280C fermentation temperature. | Investigation of fermentation conditions for Candida bombicola ACTT22214 from molasses and soybean oil for sophorolipid production