This research focused on technical assessment of GABA production from rice bran through fermentation by Lactobacillus brevis. Influence of operating pressure on separation of GABA by nanofiltration membrane was investigated and 4 bar was suitable for the nanofiltration process. The purification of GABA by nanofiltration with constant feed volume was carried out and purity of GABA reached folds, compared to feed, at 5 volumes of added water. At 40 of concentration factor in concentration of GABA solution by nanofiltration with full recycle of retentate, content of GABA reached g/L. The production of GABA from defatted rice bran at pilot scale was carried out at 1,000 L/batch (equal to 200 kg of rice bran) of fermentation. Results obtained from pilot production showed that, with 200 kg of defatted rice bran, kg of GABA powder was obtained. Results indicated that, it is potential to produce GABA from rice bran through the fermentation by Lactobacillus brevis. | Technical assessment of Gamma-amino butyric acid (GABA) production from rice bran