Nghiên cứu hiệu quả của chế phẩm sinh học phòng trừ tuyến trùng Tylenchulus semipenetrans trong đất trồng cây có múi ở Cao Phong, Hòa Bình

Nghiên cứu này đã thử nghiệm hiệu quả của chế phẩm EM, AMF, AT+Ketomium và Chitosan-Super trong phòng trừ tuyến trùng. Trong phòng thí nghiệm, tuyến trùng T. semipenetrans được tách lọc khỏi đất và kiểm tra khả năng sống sót trong môi trường dịch thể có chứa chế phẩm sinh học EM và Chitosan-Super. | Nghiên cứu hiệu quả của chế phẩm sinh học phòng trừ tuyến trùng Tylenchulus semipenetrans trong đất trồng cây có múi ở Cao Phong, Hòa Bình VNU Journal of Science: Earth and Environmental Sciences, Vol. 35, No. 4 (2019) 130-138 Original Article The Effectivity of Bioinoculants on Suppressing Tylenchulus semipenetrans in Citrus Growing Soil in Cao Phong, Hoa Binh Nguyen Thi Thao1, Trinh Quang Phap2,3, Tran Thi Tuyet Thu1,* 1 Faculty of Environmental Sciences, VNU University of Science, 334 Nguyen Trai, Hanoi, Vietnam 2 Instute of Ecology and Biological Resources, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Hanoi, Vietnam 3 Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Hanoi, Vietnam Received 15 August 2019 Revised 04 December 2019; Accepted 12 December 2019 Abstract: Tylenchulus semipenetrans causes serious damages related to decline on citrus in Cao Phong district, Hoa Binh province. This study evaluated the effects of EM, AMF, AT+Ketomium and Chitosan-Super in the control of nematodes. In the laboratory condition, the T. semipenetrans was isolated from the soil and assessed for survival in the liquid medium containing EM and Chitosan-Super. The larval mortality rate reached after 72 hours when using Chitosan-Super at 2% concentration. For pot experiments, T. semipenetrans and bioinoculants were infected into Hoa Binh red grapefruit rhizospheres. The results indicated that nematode density in the soil decreased the most in CT5 (Chitosan-Super), followed by CT4 (AT+Ketomium), CT3 (AMF+EM) and CT1 (AMF), CT2 (EM); nematode density in roots was the highest at CT5 of 132±27 individuals/5g of roots, while in CT1 there was no parasitic nematode on the red grapefruit root though its density in soil was high (±125 individuals/250g of soil). Citrus grew normally in all of the experience formulas. Research .