Pseudomonas aeruginosa is one of the commonly isolated gram negative bacillus from various clinical samples. It is a commensal in healthy individuals but can be opportunistic pathogen especially in hospitalised patients. It is intrinsically resistant to many antibiotics. Hence the present study was undertaken to study the prevalence and antibiotic sensitivity pattern in our hospital. Various clinical samples like pus, sputum, urine, vaginal swab, pleural fluid, ear swabs and tracheal aspirate received in the microbiology laboratory were included in the study. Identification and antibiotic sensitivity testing was done by standard laboratory procedures. A total of 157 Pseudomonas aeruginosa were isolated from 858 clinical samples with a prevalence rate of . Age and gender wise distribution shows higher prevalence rate in 40-60 years age group and in males. Maximum isolates were from pus, sputum and urine samples. Antibiotics tested were ceftazidime, ceftriaxone, piperacillin-tazobactum, aztreonam, amikacin, gentamycin, netilmicin, ciprofloxacin, ofloxacin, meropenem, cefixime. Antibiotic sensitivity pattern was found to show higher sensitivity to meropenem, piperacillin-tazobactum and amikacin. Resistance was more pronounced in cefixime, netilmicin, ceftriaxone and aztreonam. Spread of drug resistance in Pseudomonas aeruginosa is to be prevented by using antibiotics judiciously adhering to the antibiotic policy. | Prevalence of pseudomonas aeruginosa in various clinical samples and its antibiotic susceptibility pattern in a Tertiary care hospital