Considering lack of research on biochemical analysis of green tea manufactured by gradually increasing small tea growers of Assam and to know the changes of the phytochemicals taking place during traditional processing, the present investigation was carried out. The present investigation revealed that the phytochemicals in green tea changed significantly during processing from fresh leaves to final product. Due to processing, the constituents like ash, total chlorophyll content, total phenol, ascorbic acid and antioxidant activity in terms of DPPH scavenging decreased from to , mg/g to mg/g, % to , mg /100 g to mg/100 g and 83% to , respectively. Boiled, rolled and dried green tea samples revealed the highest content of ascorbic acid ( mg/100 g) and antioxidant activity in terms of DPPH scavenging () among four types of final products of green tea. The highest total phenol content () was found in green tea processed through boiling, rolling and pan firing and the highest ( gm/g) chlorophyll content was found in steamed, rolled and pan fired green tea.