The experiment was conducted to control the Sclerotium rolfsii causing of collar rot disease in sunflower in- vitro condition. Different fungicides, region specific bio-agents and bio-rationals were screened against the pathogen. Among the fungicides Tebuconazole gave significantly (at 5% level) highest percent inhibition of the growth in all concentrations. Among the seven bio-rationals at 10% concentration, the maximum per cent inhibition was observed in neem () wherever at 20% and 30% concentration the maximum per cent inhibition was observed in cow urine (). In the case of bio agents highest per cent inhibition of the fungal growth was obtained in T. harzianum (). | An in-vitro assay: Employing different management options for collar rot disease of sunflower caused by Sclerotium rolfsii sacc. under coastal zone of west Bengal, India